Post transcriptional regulation activity

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Developed by: Donna Koslowsky

Modifications:

Post-transcriptional Regulation: Iron-Response Element

Campbell's Biology, 9 ed, Chapter 18

Regulation of Gene Expression. Mechanisms of Post-Transcriptional Regulation. This section in Campbell Biology lists a number of ways that cells can regulate gene expression at the post-transcriptional level. The students however, have a difficult time visualizing how some of these mechanisms may work.  This activity was designed to help them visualize an important aspect of post-transcriptional control: regulating protein levels by both regulating translation and by regulating mRNA half-life.    

Connection to Vision and Change

Core Concepts

3. INFORMATION FLOW, EXCHANGE , AND STORAGE :

The growth and behavior of organisms are activated through the expression of genetic information in context.

Core Competencies and Disciplinary Practice

3. ABILITY TO USE MODELING AND SIMULATION: 

Biology focuses on the study of complex systems.

Objectives

  • Identify and describe several different ways that gene expression can be controlled Post-transcriptionally.
  • Based on the model, explain how different iron conditions regulate translation and mRNA stability.

Description

At the beginning of class, students are given a Handout which describes each of the different molecules and elements involved in iron storage and transport in a eukaryotic cell and a Table.  A brief (5-10 minute) mini-lectures is given on key aspects of Post-transcriptional gene expression.  Students are then told to work together to complete the table given to them with the handout.  Completion of the Table is assessed by a series of clicker questions.  Clicker questions were designed to both link earlier key concepts to the current topic and to determine how well they understand the system.

AttachmentSize
IRE handout for students 155.72 KB
AttachmentSize
Activity and clicker slides ONLY146.99 KB
AttachmentSize
Full lecture including activity and clicker slides3.01 MB

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This material is based upon work supported by the National Science Foundation (DUE grants: 1438739, 1323162, 1347740, 0736952 and 1022653). Any opinions, findings and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the NSF.